The Baker Heart and Diabetes Institute Metabolomics Laboratory has developed a state-of-the-art lipidomics platform, emphasizing precise and reliable measurement of a broad range of lipid species from human plasma. This website describes the targeted LC-MS/MS method for the determination of 761 different human lipid species using just 10 µL of plasma. The total analysis time is 16 minutes per sample using a single column, reduced to 13 minutes when running a dual column system.
Our platform has been built from the ground up to separate isomers and isobaric species, allowing an unparalleled specificity in lipid measurement. We have developed a freely available online resource detailing our lipidomic methodologies. This resoure will allow the research community to replicate our lipidomic platform.